dc.contributor |
Graduate Program in Chemistry. |
|
dc.contributor.advisor |
Sanyal, Amitav. |
|
dc.contributor.author |
Gevrek, Tuğçe Nihal. |
|
dc.date.accessioned |
2023-03-16T11:01:44Z |
|
dc.date.available |
2023-03-16T11:01:44Z |
|
dc.date.issued |
2011. |
|
dc.identifier.other |
CHEM 2011 G48 |
|
dc.identifier.uri |
http://digitalarchive.boun.edu.tr/handle/123456789/14485 |
|
dc.description.abstract |
PEG-based 3D hydrogel patterns containing thiol reactive maleimide functional groups have been prepared using a novel Diels-Alder/retro Diels-Alder strategy. Bulk-hydrogels and hydrogel patterns containing various amount of thiol reactive maleimide were synthesized by photo-polymerization in presence of photoinitiator and PEG-DA cross linker. The thiol reactive maleimide group was protected with furan during gelation of bulk-hydrogel and patterning of hydrogels and then was deprotected by retro-Diels-Alder reaction. After activation of maleimide in the hydrogel structure, the reactive maleimide group embedded hydrogels can be efficiently derivatized with thiol containing molecules such as a fluorescent dye, BodipyC10SH. Thiol containing biotin derivatives were covalently attached to the thiol reactive maleimide in bulk-hydrogel and hydrogel patterns under mild condition. Immobilization of FITC-streptavidin onto biotinylated hydrogel patterns were performed and investigated by fluorescence microscope. In order to detect amount of accessible biotin in the hydrogel, HABA-assay was carried out with biotinylated bulk-hydrogels. |
|
dc.format.extent |
30 cm. |
|
dc.publisher |
Thesis (M.S.)-Bogazici University. Institute for Graduate Studies in Science and Engineering, 2011. |
|
dc.relation |
Includes appendices. |
|
dc.relation |
Includes appendices. |
|
dc.subject.lcsh |
Thiols. |
|
dc.subject.lcsh |
Reactive polymers. |
|
dc.title |
Fabrication of thiol reactive polymeric microstructures |
|
dc.format.pages |
xii, 44 leaves ; |
|