Abstract:
Optimization of nanofiber (NF) surface properties is critical to achieve an adequate cell response. Here, the impact of conjugation of biomimetic aspartic (ASP) and glutamic acid (GLU) templated peptides with PLGA electrospun NF on osteogenic differentiation of bone marrow stromal cells (BMSCs) was evaluated. Cold atmospheric plasma (CAP) was used to functionalize the NF surface and thus to mediate the conjugation. The influence of the CAP treatment following with peptide conjugation to the NF surface was assessed using scanning electron microscopy (SEM), contact angle measurements, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), Both the hydrophilicity of NFs and the number of the -COOH groupsonthesurfaceincreasedwithrespecttothedurationofCAPtreatment. Results demonstrated that CAP treatment significantly enhanced peptide conjugation on surface of NF. Osteogenic differentiation results indicated that conjugating of biomimetic ASP templated peptides sharply increased alkaline phosphatase (ALP) activity, calcium content, and expression of key osteogenic markers of collagen type I, osteocalcin, and osteopontin compare to GLU conjugated and neat NF. It was further depicted that ASP sequences are the major fragments that influence mineralization and osteogenic differentiation in non-collagenous proteins of bone extracellular matrix.|Keywords : Bone tissue engineering, Peptide, Scaffold, Surface functionalization.
